Permaculture Blog

I have been recently pondering some of the “information” that is being espoused on the internet. This “information” — internet, books, and social media, YouTubes — question the existence of viruses.

I believe that we should always question what we believe. However, I would also like to address the idea of lying by omission.

The most important piece of information to realize here is that there are three major cell types. Two of them are ca. bacteria sized, (bacteria, and archaea) and one is eukaryotic (a thousand times more massive then bacteria). Human beings, and all animals that are visible by naked eye are built out of these huge, eukaryotic cells.

All three of these cell types reportedly have “viruses” that prey on them. But I would like to focus here on the _bacterial_ “viruses”.

Bacteria are physically tougher than eukaryotic cells. They are far, far easier to grow, manipulate, and experiment on. There is a product called agar. (please look up any of these terms. That’s why I provide them. But I am not going to waste space detailing information about them because so many of you already know it.) Agar is similar to a thick, firm, Jello and must be heated to boiling to melt but it then does not resolidify until it’s just hot. A hot bath temp that you can barely tolerate but you can tolerate. Once at that “just hot” point it solidifies quickly.

I can take a solution of bacteria approximately 3 * 10e9 (three times 10 to the exponent of 9) or roughly three _billion_ cells and mix that with several volumes of hot – not boiling – agar, mix, and then quickly pour it into a petri plate where it will instantly cool and solidify. The mixture I poured it into the plate is cloudy with bacteria that are still alive. There are billions of them suspended. They form a faint cloud in the agar that forms a firm gel in the bottom of the Petri plate. I can then save that plate and others that I have made in the refrigerator for future work.

Here is where the viruses come in. I have been exposed to thousands of books that show information on the life styles of bacterial viruses, electron micrograph images, DNA sequences, and specific mutants of these viruses. I have studied experiments that allowed us to figure out how certain protein and enzyme systems in these bacterial viruses work, even how different DNA regulation systems work. All this information about bacterial virus systems, has led to some of our beliefs of what might be properties of human viruses.

All of this information about bacterial viruses allows them to be used in some cases as tools to explore other mechanisms. And that is what I wanted to do in my lab work. I wanted to create a tool that would allow me to study how systems interact with our biological membranes. I did not care in the least about studying bacterial viruses😀 I simply wanted to use them as a tool.

I wanted to mutagenize some bacterial virus to create this tool. So I took some “virus” material from a lab stock. A little bit of tea colored liquid. And I wanted to know how many virus particles there were in it. I had been taught that there were probably in the neighborhood of 10 to the 10th (1x10e10) virus particles. But that it might vary. I needed to actually count what was in this particular sample.

I diluted 10 microliters (about 1/5 of a small drop) into one milliliter of liquid (a 1/100 dilution [-2].) I got a clean transfer tip and took 10 microliters of that dilution into another mil [-4], and again [-6]. In theory I am down to 1e4 “virus” particles. I now do a last dilution of only a 1 to 10 instead of 1 to 100. In theory I now have a solution that has about a hundred virus particles in 100 microliters.

Here’s where the plates that I made earlier come in. Supposedly, these virus particles will infect bacterial cells. So I grab one of my bacteria agar plates. And I spread 100 microliters of this last dilution or possibly ca.100 virus particles on the surface of that plate.

In theory the virus particles will bump up against the embedded cells attach and infect. Or, in the worst case scenario vibrate around in the water for a little bit (there are billions of bacterial cells) so it can’t go very far before it bumps into a cell and infects it. Virtually all the virus particles quickly end up bumping in the cells and infecting them.

After I let this “grow” overnight, I get a very cloudy plate because all the uninfected bacteria have continued to grow in their little jello places. Whereas the cells that were infected made lots more virus and popped, releasing virus particles, that infected the cells around them, made more, popped them, and so on, and so on killing all the cells in a little area around the original virus particle. So you get little clearish areas about the diameter of a pinhead scattered around the plate where there was a virus particle originally. These little clearish areas are called plaques. And you can _count them_.

I can count the number of particles I put on the plate. I can calculate the number of particles in that original stock solution. These particles caused little clearish holes in my bacterial lawn. I can think of no possible way that a chemical poison could do this.

I can take a type of lab chemical and show in many ways that it is a mutagen. Literally hundreds of thousands of people currently doing research in the United States can as well. And I can treat a solution of virus particles with this chemical and create virus mutants that instead of clearish plaques create clear, crystal clear, plaques.

I see no possible way that I could mutagenize a _chemical_. The most intelligent, straightforward, and simple explanation to me is that study after study after study are correct and that these are bacterial viruses. And the idea that these bacterial viruses do not exist is like me holding out a ball and dropping it and expecting it to go _up_.

I can write about experimental cases where the ball _does_ go up, and I can enthusiastically try to convince other people that that means that balls always go up and accidentally (or conveniently) leave out the fact that I am aboard the international space station under thrust.

However; that would _not_ be the honest thing for me to do. What we must start doing today is calling out the authors, politicians, and especially pesticide manufacturing PR departments that leave out _the important stuff_; intentionally, or conveniently, and thus lie by omission. But it’s hard to be sure whether it was deliberate or not. Accidents do happen. Sometimes authors don’t do it deliberately, and conveniently, but unfortunately just ignorantly.

Eukaryotic viruses are hundreds or even thousands of times more difficult then bacteria to work with. They cannot be analyzed and made into tools as easily as I just described. And whether we have identified the correct human virus for a particular disease is certainly more questionable than whether eukaryotic viruses exist.

Questioning whether we have the right policy for over population is _not_ the same as questioning whether the Earth is flat, or round, or whether people even exist. Please read carefully and think!

I would encourage all of you to not shoot yourself in the foot by simply believing that all viruses don’t exist.

The absolute best to you all!

For past OTNP (#_OTNP on X) issues.
https://www.puravidaaquatic.com/wordpress/past-ourtoxicnationalparks-posts/

But you may want to start here. www.puravidaaquatic.com/wordpress/why-i-started-this/

If you have received this as a forward and would like to continue receiving it please email me “vidaaquatic@gmail.com”
And if you would like to donate as little as $5 to the cause, you can Zelle it to vidaaquatic@gmail.com. thank you very much. Bob

OTNP September 2024

Good morning!😀 Hope you all are having a wonderful month!
It is so easy to complain 😀 about so many things these days. Unfortunately most times the complaints have no real doable solution other than “change everything about government.” I believe that there is a _doable_ solution to our pesticide problems. I think there are three things, that are very easy, that we can do.😀 That will make things dramatically better for future generations. Just three easy steps

Elephants were not destroyed by pesticides … just thoughtless behavior and destruction of natural areas. Same behavior our US national parks are showing today

dimethyl tetrachloroterephthalate is in the news these days. As the EPA is banning it after 65 years on the market. 65 years of the same side effects and damage to the environment that all of a sudden is now important to ban. We cannot continue to ban individual pesticides. We must and can start safe zones.

https://en.wikipedia.org/wiki/Dimethyl_tetrachloroterephthalate#:~:text=roughly%2039%25%20of%20the%20fish%20tested%20had%20stored%20DCPA%20concentrations%20that%20exceeded%20their%20limit%20of%20detection%2C%20and%20the%20male%20fish%20had%20higher%20stored%20concentrations%20of%20DCPA%20than%20their%20female%20counterparts.%5B14

Roughly 39% of the fish tested had stored DCPA concentrations that exceeded their limit of detection, and the male fish had higher stored concentrations of DCPA than their female counterparts.[14″

That last line is interesting! DCPA has a different affinity for male versus female. I wonder how many other toxins we are spraying around have different affinities depending on whether you or your children are male or female? 🤔😀 And since most pesticides haven’t even been remotely tested in this respect, this is another example of why we need safe zones. We spent 10 years working to get Roundup banned. And we didn’t even get it banned. In the same 10 years hundreds of new pesticide products have been approved. And the adjuvants that are mixed with them are mostly not regulated (approved) at all.

the EPA said exposure to the pesticide, dimethyl tetrachloroterephthalate, also known DCPA or dacthal, can affect fetal thyroid hormone levels. These changes are in turn associated with lower birth weights and impairments to brain development and motor skills

Another source:

Studies have demonstrated that DCPA acts as a chemical disruptor by interfering with microtubule formation in exposed cells. This interference results in abnormal cell division. The abnormal microtubules affect cell wall formation as well as chromosome replication and division.[20] The key difference between DCPA and other mitotic inhibitors is that it often produces multinucleate cells.[21] It essentially kills plants by inhibiting cell division in this manner.[20]

Another source:

EPA said it consulted with the U.S. Department of Agriculture (USDA) on “alternatives to this pesticide,” and presumably determined that there were “alternative chemicals” that could be used in chemical-intensive agriculture.—while not considering “alternatives to chemicals.” This is the framework that is understood to be EPA’s process that keeps pest management on a pesticide treadmill

https://beyondpesticides.org/dailynewsblog/2024/08/elevating-the-urgent-need-to-take-action-drawing-on-the-epas-emergency-ban-of-the-weed-killer-dacthal-dcpa/#:~:text=EPA%20said%20it,a%20pesticide%20treadmill

Our Toxic National Parks will not tell you if they are spraying DCPA in our parks and on our endangered species.

We must get our legacy conservation groups involved and we must generate safe zones where all toxic chemical use is prohibited.  I started to say indiscriminate or careless chemical use above but then I realized that our big PR departments would argue that the use of xxxx under “carefully controlled conditions”  isn’t careless or indiscriminate. Even though by their understanding of carefully controlled conditions, it actually is.

There are more than 3,000 types of neurons. These neurons govern what we think of as our personality; language, social skills, empathy, and almost certainly hatred, jealousy, entitlement, animosity, and even certain social ills too. It certainly would not be impossible from my biological point of view that some of the pesticides and adjuvents being sprayed could effect these neuron types differently. The wars, political animosity, and even certain social ills of today could certainly be, in part, due to our increasing chemically toxic world, and not just people’s individual choices.

And if you think that because you get organic fruit and vegetables for your children that they are better off…. They actually may be more exposed 😮 Because adjuvants are unregulated.
https://www.puravidaaquatic.com/wordpress/pfas-widely-added-to-us-pesticides-despite-epa-denial-study-finds/

I believe we _must_ create safe zones.

The sierra club, world wildlife fund, and audubon society are well aware of the issues that I am regularly presenting. They are well aware of the PFOS contamination of pesticides and adjuvants. Chemicals used even for organic crops.🤔 I would assume they can figure out the danger these toxins represent to our native endangered animals. Until they create programs and publish pesticide use statistics from our _wilderness areas_, I will continue to consider them simply _enablers_ of the pesticide industry, and I believe you should as well.
Three little easy things

For past OTNP issues.
https://www.puravidaaquatic.com/wordpress/past-ourtoxicnationalparks-posts/

@vidaaquatic on X

But you may want to start here. www.puravidaaquatic.com/wordpress/why-i-started-this/

If you have received this as a forward and would like to continue receiving it please email me “vidaaquatic@gmail.com”
And if you would like to donate as little as $5 to the cause, you can Zelle it to vidaaquatic@gmail.com. thank you very much. Bob

One of my best friends is very, very smart. But also very very opinionated.😀 I was trying to describe a complex biological process and he says “I don’t care” and walks away. Now he’s very very good at changing his opinion when he gets a new viewpoint. And that is why we are great friends. If he would make the effort to understand the process I was trying to describe I can guarantee that he would change his mind. But he can’t _yet_.😀

I would like to try to describe a biological system that some of you may be well aware of but others may be not. How do we identify carcinogens?

The newspapers tell you that science has decreed a certain chemical as “likely to be a carcinogen.” How does the news media’s version of “science” do that?🤔

It’s called the Ames test. And what Bruce Ames wanted to do was find a way to detect mutations. He took a strain of bacteria that could grow fine with basically sugar and nitrogen and some mineral nutrients. And then he damaged it’s ability to make an amino acid -histidine. Now this bacterial strain can only grow if you supply it with histidine.

Bacteria and our cells always have a background rate of mutation. If you take a drop of Ames’s creation (about 3X10^8 cells) 100 million! cells and spread it around on a petri plate with the basic nutrients and _no histidine_ the vast majority of the cells can’t grow however five or six cells will grow. These cells grow and grow, overnight all in one place, and become visible colonies. Five or six cells will form colonies in this case, because that is the normal rate at which mutations are occurring in 100 million cells.

You can count those colonies and count the mutation frequency. I’m trying to keep this simple and just give the idea behind it so don’t get too tangled up with numbers.

I googled and tried to find a “simple description of the Ames test” and unbelievably I couldn’t find one that I didn’t think was just way too complicated. But you can certainly Google it online. (Auxotrophs and heterotrophs geez what’s wrong with people 😀)

Now the DNA changes that make the bacterial strain unable to make histidine are _very specific_ and this is why Bruce Ames is given so much credit for developing it. There are a set of strains. Some strains have simple DNA changes and others more complex ones. But the general point is that if the cells are exposed to a mutagen and then (as above) 100 million cells are plated; 10, 100, even a thousand cells exposed to this mutagen will have been mutagenized and will form colonies just like above and can be counted.

At the lower end where it’s very close to normal background is where the fuzziness (maybe it is maybe it’s not) comes in. But if a thousand colonies pop up it’s definitely a mutagen. Most carcinogens turn out to be mutagens. if you get a thousand colonies the substance is designated a likely carcinogen.

There’s one additional trick. Our livers are trying to DEtoxify environmental chemicals. Unfortunately since they have not evolved with the latest greatest toxin they often metabolize today’s chemicals and drugs into problems. Mouse liver extracts metabolize chemicals as our livers would. And so to be sure that these liver breakdown products aren’t themselves carcinogens, liver extracts are required in the process. Turns out that there are a lot of cases where the original chemical was not a carcinogen but after being exposed to what our livers can do to it the chemical was turned into a carcinogen.😮

And here is my conspiracy theory 😀. 30 years ago my wife was in a plant research lab and started talking about some of her old research that was with plant extracts (like the liver extracts above) metabolizing common pesticides into carcinogens. I was interested and got reprints from her. Unfortunately I lost them when I lost my house. And, they are now gone from the internet. 😮 But the papers I read showed that plant extracts which had not been studied previously could metabolize common pesticides into carcinogens. I can’t find hide nor hair of the papers.🤔
Always the best to you all!

http://www.puravidaaquatic.com/

I have just made an excellent contact at
Wildlife Diversity Program
California Department of Fish & Wildlife
They say that they will forward emails supporting the protection of bumblebees to the committee as part of the official record. I am not going to put their contact out on the web. But if you would care to email any support at all for the bumblebees to me (if it’s kind and at all reasonable) I will forward it
Additionally, they say that

there will be several opportunities for public comment during Fish & Game Commission meetings when CDFW provides the status review to the Commission and they consider it (2 separate meetings).

I will try to get them to send me a notification when those opportunities for public response are made and will edit below that information.
The best to everyone.